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yitB [2018-02-10T12:20:23.000Z]
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yitB [2018-02-10T12:20:23.000Z]

phospho-adenylylsulfate sulfotransferase
Locus
BSU10930
Isoelectric point
5.77
Molecular weight
27.52 kDa
Protein length
236 aa Sequence Blast
Gene length
708 bp Sequence Blast
Function
sulfate reduction and activation
Product
phospho-adenylylsulfate sulfotransferase
Essential
no
E.C.
1.8.4.8
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,171,755 → 1,172,465

The protein

Catalyzed reaction/ biological activity

  • Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin (according to Swiss-Prot)
  • Protein family

  • CysH subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

    Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon
  • Regulation

  • expressed during sporulation in the mother cell (SigK) PubMed
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    Biological materials

    Mutant

  • MGNA-A504 (yitB::erm), available at the NBRP B. subtilis, Japan
  • BKE10930 (ΔyitB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG, downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG
  • BKK10930 (ΔyitB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG, downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG
  • References

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